Suzhou Yacoo Science Corporation was founded in 2003, specialized in Biobuffer, Diagnostic reagents, Medical intermediates and Electronic chemicals. We have our factory in Shandong Province to offer high-quality and low-price HEPES for Yacoo customers.
Specification:
English Name：4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
Molecular Formula：C8H18N2O4S
Molecular Wt：238.31
CAS：7365-45-9
Shelf life: two years
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SPECIFICATION PROPERTIES
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STANDARD
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Appearance
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White crystalline powder
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Assay,%
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99.0～101.0
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Solubility (1M aq.)
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Clear, colorless solution
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PH (1% aq.)
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4.7-6.0
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Heavy Metals,ppm
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≤5
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Loss on drying,%
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≤0.5
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Ultraviolet absorbance（1 M aq.）
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≤0.065
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Ultraviolet absorbance（1 M aq.）
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≤0.055
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Iron ion, ppm
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≤5
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Sulfate Ion,ppm
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≤10
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Ignition residue,%
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≤0.1
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Usage: HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES is like water in that its dissociation decreases as the temperature decreases. This makes HEPES a more effective buffering agent for maintaining enzyme structure and function at low temperatures. Lepe-Zuniga et al. reported a phototoxicity of HEPES when exposed to ambient light by the production of hydrogen peroxide, which is not a problem in bicarbonate-based cell culture buffers. It is therefore strongly advised to keep HEPES-containing solutions in darkness as much as possible.
Storage：room temperature
Packing: 25kg/Drum, or as your request
