99.5% Tris(hydroxymethyl)aminomethane CAS 77-86-1

Tris(hydroxymethyl)aminomethane, also known as THAM, is widely used as a biological buffer or as a component of buffer formulations such as TAE and TBE buffers. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.


  • AppearanceWhite crystalline powder
  • Assay≥99.5%
  • Weight121.135
  • FormulaC4H11NO3
  • CAS No77-86-1

Specification:


English Name:Tris (hydroxymethyl) aminomethane

Molecular Formula:C4H11NO3

Molecular Wt:121.14

CAS:77-86-1

Shelf life:two years

SPECIFICATION  PROPERTIES

STANDARD

Appearance

White crystalline powder

Assay,%

≥99.5

MP/℃

168.0-171.0

Solubility(40% aq.)

Clear, colorless solution

Heavy Metals, ppm

≤5

Water Content,%

≤0.2

PH value(5% aq.)

10.0-11.5

Iron ion,ppm

≤1

Sulfate,ppm

≤10

Chloride,ppm

≤3

Ultraviolet absorbance(40% aq.)

≤0.07 

Ultraviolet absorbance(40% aq.)

   ≤0.02

Infrared spectrum

Conforms to reference


Usage:


1, Tris buffer, only nucleic acids and proteins are widely used as solvents.
2, Tris is used for protein crystal growth under different pH conditions.
3, Tris buffer having a low ionic strength can be used nematode (C. elegans) is formed lamin (lamin) intermediate fibers.
4, Tris is a major component of protein electrophoresis buffer.
5, Tris for the preparation of a surfactant, a vulcanization accelerator, and some drug intermediates.
6, Tris titrated also used as standard.


Storage:room temperature

Packing: 25kg/Drum, or as your request

Tris(hydroxymethyl)aminomethane Buffer Supplier



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