Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling)

1. Product Information

Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) provides a fast measurement for endotoxin quantification. The endotoxin in the sample activates a cascade of enzymes in Amebocyte Lysate, the activated enzyme splits the synthetic substrate, releasing a yellow-colored item. Then the yellow item can further react with diazo reagents to form purple items with maximum absorbance at 545nm. A regular spectrophotometer or plate reader is required for the assay. The purple items are proportional to endotoxin concentration. Then endotoxin is quantified.

2. Product Parameter

Sensitivity Range: 0.01-0.1EU/ml (assay time about 46 minutes)

                             0.1-1.0EU/ml (assay time about 16 minutes)

3. Product Features and Application

Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling) is intended for use in the In Vitro detection and quantitation of gram-negative bacterial endotoxins. The colorless artificial peptide substrate solution is added into Lyophilized Amebocyte Lysate, and then test specimen mixture. If specimen contains endotoxin, color of specimen mixture changes. The absorbance is related to the endotoxin concentration. Therefore the endotoxn levels in specimen mixture can be claculated against a standard curve. Standard spectrophotometer with 540 - 545nm filter is enough to quantify endotoxin with our EC Endotoxin Test Kit (End-point Chromogenic Assay, Diazo Coupling).

Note:

Lyophilized Amebocyte Lysate reagent manufactured by Bioendo is made from amebocyte lysate from the horseshoe crab (Tachypleus tridentatus).