The inactivated preservation solution is a kind of virus preservation solution, which is mainly used to store the virus after the inactivation of the virus, and the inactivated virus preservation solution can effectively prevent the second infection of the user. Its characteristic is that it can quickly inactivate the virus, degrade the viral protein membrane capsid, and release the viral nucleic acid. At present, the new coronavirus that is sweeping the world in society is to release and degrade RNA viruses, so that post-reverse transcription PCR amplification experiments can be performed to achieve the purpose of using nucleic acid to detect viruses.
In addition to the lysing components, the inactivated virus preservation solution usually contains detergents that denature proteins and destroy the membrane structure, biological buffers, inhibitors that prevent nucleases from degrading nucleic acids, and reagents that maintain the stability of the nucleic acid structure. The lysis system may also add protease to cut the protein into small fragments to promote the separation of protein and nucleic acid.
At present, there are many known methods for lysing viruses. Two of the more common methods are heating method and concentrated salt method:
The concentrated salt method uses high concentration of salt to destroy the secondary bond between nucleic acid and protein, disassociating the nucleoprotein and releasing the viral nucleic acid. Usually, guanidine hydrochloride, guanidine isothiocyanate or non-guanidine salt cleavage salt can be used to directly lyse and inactivate the virus without heating the sample, which is relatively simple.
The heating method is based on heating to a temperature value of 80 C ~ 100 C, because generally samples are frozen at -70 C, generally need to be heated for 5 to 10 minutes, this operation is relatively simple, only one step can be used for The template for nucleic acid PCR amplification does not require any operation steps such as centrifugation and extraction, and is faster than the commonly used CheleX100 extraction method and phenol/chloroform methods. Although this method is simple, the amount of nucleic acid extracted will be relatively small.
Since the outbreak of the epidemic, Hubei Xindesheng Materials Co., Ltd. has been devoted to the research and development of virus preservation solutions, striving to provide society with its own strength. After many experiments and tests, Desheng has been able to ensure that the product quality and experience are very up to standard. Among them, our company produces inactivated and non-inactivated virus preservation solutions, which can be adjusted and selected on demand. In addition, Desheng reminds our customers that when purchasing virus preservation solutions, you should not blindly pursue low prices. You can take samples separately and go back for testing and comparison. The best is the best.